South African Avocado Growers’ Association Yearbook 1986. 9:75-77 RECENT EXPERIENCE WITH THE USE OF SYNTHETIC DNA PROBES FOR THE DETECTION OF AVOCADO SUNBLOTCH VIROID
نویسندگان
چکیده
Recent experience using 23 oligonucleotides to diagnose avocado sunblotch viroid (ASBV) is described. In a number of field collected samples with sunblotch symptoms, the probe was unsuccessful in detecting the viroid. The sunblotch viroid varies greatly from branch to branch in a tree. Tree to tree variation is also common. However, the probe can be used with a reasonable measure of success to detect sunblotch infected avocado trees.
منابع مشابه
Self-cleavage of plus and minus RNA transcripts of avocado sunblotch viroid.
Self-cleavage of both plus and minus RNA transcripts of the 247-residue avocado sunblotch viroid (ASBV), prepared from tandem dimeric cDNA clones, occurs specifically at two sites in each transcript to give monomeric plus and minus species. The cleavage reaction occurs both during transcription and on incubation of purified transcripts at pH 8 and 37 degrees C in the presence of magnesium ions ...
متن کاملSouth African Avocado Growers’ Association Yearbook 1991. 14:67-71 DEFICIENCY SYMPTOMS IN AVOCADOS
Colour photographs of typical nutrient deficiency symptoms in avocados, obtained in water and sand culture under carefully controlled conditions, are presented.
متن کاملApplication of a Highly Sensitive Avocado Sunblotch Viroid Indexing Method
In South Africa, ASBV indexing techniques such as biological indexing, polyacrylamide gel electrophoresis, radiolabeled oligonucleotide probes and DNA and RNA hybridisation with digoxigenin (DIG) labeled probes have been used over the years. Testing became increasingly more sensitive and less time consuming, but some known positives were still missed due to the low viroid titre. Our objective w...
متن کاملThe 5' end generated in the in vitro self-cleavage reaction of avocado sunblotch viroid RNAs is present in naturally occurring linear viroid molecules.
The 5' termini of linear forms of avocado sunblotch viroid (ASBVd) isolated from infected tissue have been determined by primer extension analyses. According to the different termini identified, four main populations of linear viroid molecules were found. One of them had the same 5' end as the one produced in the in vitro self-cleavage reaction of ASBVd dimeric transcripts mediated by a hammerh...
متن کاملA chloroplast protein binds a viroid RNA in vivo and facilitates its hammerhead-mediated self-cleavage.
Viroids, small single-stranded circular RNAs (246-401 nucleotides), do not have mRNA capacity and must recruit host proteins to assist in the steps of their biological cycle. The nature of these cellular factors is poorly understood due to a lack of reliable experimental approaches. Here, to screen for host proteins interacting with viroid RNAs in vivo, we UV-irradiated avocado leaves infected ...
متن کامل